Journal: Molecules

Article Title: Inhibition of MORC2 Mediates HDAC4 to Promote Cellular Senescence through p53/p21 Signaling Axis

doi: 10.3390/molecules27196247

Figure Lengend Snippet: Knockdown of MORC2 inhibits HCT-116 and Lovo cell proliferation and clone formation. ( A ) GSEA indicates that MORC2-associated DEGs were enriched in the cell cycle. ( B ) HCT-116 and Lovo cells were transfected with indicated shRNA, and the protein expression was analyzed with indicated antibodies. ( C ) CCK8 assays were used to assess the DNA synthesis of shCON, shMORC2#1, and shMORC2#2 groups with HCT-116 and Lovo cells. ( D , E ) Edu assay was used to assess the DNA synthesis of shCON, shMORC2#1, and shMORC2#2 groups with HCT-116 cells. The percentage of EdU-positive cells in shCON, shMORC2#1, and shMORC2#2 groups. ( F ) Colony formation assay showing clone formation capacity of shCON, shMORC2#1, and shMORC2#2 groups. ( G ) Each group colony number was counted and relative colony numbers in shMORC2#1 and shMORC2#2 were compared to the shCON group. ** p < 0.01, **** p < 0.0001.

Article Snippet: HCT-116 cells were obtained from Procell Co., Ltd. HCT-116 and HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), under 37 °C and 5% CO 2 conditions.

Techniques: Transfection, shRNA, Expressing, DNA Synthesis, EdU Assay, Colony Assay

Journal: Molecules

Article Title: Inhibition of MORC2 Mediates HDAC4 to Promote Cellular Senescence through p53/p21 Signaling Axis

doi: 10.3390/molecules27196247

Figure Lengend Snippet: Knockdown of MORC2 induces cell senescence. ( A ) PPI network of the top 10 interacting genes related to MORC2. ( B ) GSEA indicates that MORC2-associated DEGs were enriched in cell senescence. ( C ) The single gene co-expression heat map presented the significant relationship among MORC2, HDAC4, IL-1α, and IL-1β. ( D ) HCT-116 and Lovo cells are transfected with shMORC2, and the protein expression is analyzed with indicated antibodies. ( E ) p53 promoter-luciferase activity was measured. ( F ) mRNA expression of p16, IL-1α, and IL-1β in HCT-116 cells. ( G – J ) The levels of IL-6 and IL-8 secreted by HCT-116 and Lovo cells after transfecting with shMORC2. ( K ) SA-β-gal staining analysis in HCT-116 and Lovo cells transfected with shMORC2. ( L ) Relative fold change of the SA-β-gal staining ratio in each shMORC2 group was calculated and compared to shCON group’s SA-β-gal staining ratio. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: HCT-116 cells were obtained from Procell Co., Ltd. HCT-116 and HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), under 37 °C and 5% CO 2 conditions.

Techniques: Expressing, Transfection, Luciferase, Activity Assay, Staining

Journal: Molecules

Article Title: Inhibition of MORC2 Mediates HDAC4 to Promote Cellular Senescence through p53/p21 Signaling Axis

doi: 10.3390/molecules27196247

Figure Lengend Snippet: Knockdown of MORC2 inhibits tumor growth. ( A ) The growth curve of mice xenografts formed by shCON and shMORC2#1 cells ( n = 5). ( B ) Tumor weights after sacrificing the mice ( n = 5). ( C ) Mice xenografts formed by HCT-116 with or without MORC2 intervention. ( D , E ) IHC staining and statistical analysis of xenografts using antibodies against ki67, MORC2, p53, and HDAC4. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: HCT-116 cells were obtained from Procell Co., Ltd. HCT-116 and HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), under 37 °C and 5% CO 2 conditions.

Techniques: Immunohistochemistry